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Cloning and Expression
Enzymes
BamHI
Supplier Images
BamHI
BamHI from New England Biolabs
Be the first to write a review!
Citations:
(125)
Supplier Page
Supplier Page from
New England Biolabs for
BamHI
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
BamHI
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
R0136S
Concentration
20-100 u/µL
Quantity
10000 units
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Restriction Enzyme
Temperature
-20 C
Sequence
G/GATCC
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (125)
(1)
Protocol to capture transcription factor-mediated 3D chromatin interactions using affinity tag-based BL-HiChIP
STAR Protocols
December 1, 2023
Ruimin Ren, Yao Hua, Heng Wang
(BSA),Takara,Cat#2320 λ-exonuclease,
NEB
,Cat#
M0262S
Exonuclease Ⅰ,NEB,Cat#M0293V NheⅠ,
NEB
,Cat#
R3131S
BamHⅠ,
NEB
,Cat#
R0136S
I-Block Protein-Based Blocking Reagent
,
Invitrogen
,Cat#
T2015
Dynabeads MyOne Streptavidin C1
,
Invitrogen
,Cat#
65001
KAPA pure beads
(2)
Activation of Csm6 ribonuclease by cyclic nucleotide binding: in an emergency, twist to open
Nucleic Acids Research
October 27, 2023
Stuart McQuarrie, Januka S Athukoralage, Stephen A McMahon, Shirley Graham, Katrin Ackermann, Bela E Bode, Malcolm F White, Tracey M Gloster
Inc.). Reagents
NcoI
and
BamHI
restriction enzymes (
New England Biolabs
, Hitchin, Herts, UK, cat. no.
R3193S
and
R0136S
respectively);
QuikChange Site-Directed Mutagenesis kit
(
Agilent Technologies
, Santa Clara, CA 95051,
(3)
A histological protocol for quantifying the birthrates of specific subtypes of olfactory sensory neurons in mice
STAR Protocols
September 1, 2023
Kawsar Hossain, Madeline Smith, Stephen W. Santoro
H1000
Dimethyl sulfoxide,Alfa Aesar,Cat# J66650
EcoRI
,
NEB
,Cat#
R0101S
HindIII
,
NEB
,Cat#
R0104S
BamHI
,
NEB
,Cat#
R0136S
NotI
,
NEB
,Cat#
R0189S
T7 RNA polymerase
,
NEB
,Cat#
M0251L
Sp6 RNA polymerase
,
NEB
,Cat#
M0207S
RQ1 RNase-free DNase
(4)
DNA methylation at the suppressor of cytokine signaling 3 (SOCS3) gene influences height in childhood
Nat Commun
August 25, 2023
Prachand Issarapu, Manisha Arumalla, Hannah R. Elliott, Suraj S. Nongmaithem, Alagu Sankareswaran, Modupeh Betts, Sara Sajjadi, Noah J. Kessler, Swati Bayyana, Sohail R. Mansuri,
et al.
vector (Supplementary Fig. 4) using
ApaI
and
BamHI
restriction enzymes (
NEB
, Catalog no.
R0114S
and
R0136S
, respectively). The vector harbors no CpG dinucleotides making it suitable for measuring the effect
(5)
Genome-wide CRISPR-Cas9 screen analyzed by SLIDER identifies network of repressor complexes that regulate TRIM24
iScience
July 21, 2023
Patel LR, Stratton SA, McLaughlin M, Krause P, Allton K, Rivas AL, Barbosa D, Hart T, Barton MC.
EDTA
,
Gibco
,25300-054 FBS,Gibco,A31605 DMSO,
Sigma
,
D8418
T4 PNK,
NEB
,
M0201S
CutSmart Buffer
,
NEB
,B7204
Esp3I
,
Thermo
,
ER0452
EcoRI
,
NEB
,
R0101S
BamHI
,
NEB
,
R0136S
T4 Ligase,
NEB
,
M0202S
XbaI
,
NEB
,
R0145S
HindIII
,
NEB
,
R0104S
Q5 Site Direct
Mutagenesis
Kit,NEB,E0554 Herculase
(6)
Escherichia coli B-Strains Are Intrinsically Resistant to Colistin and Not Suitable for Characterization and Identification of mcr Genes
Microbiology Spectrum
June 15, 2023
Anna Schumann, Alexa R. Cohn, Ahmed Gaballa, Martin Wiedmann
#69663) were digested with restriction enzymes
NdeI
(
NEB
, cat. number
R0111S
) and
BamHI
(
NEB
, cat. number
R0136S
), purified, and ligated with
T4 DNA ligase
(
NEB
, cat. number
M0202L
) according to the manufacturer’s
(7)
The RNA export factor TbMex67 connects transcription and RNA export in Trypanosoma brucei and sets boundaries for RNA polymerase I
Nucleic Acids Research
June 9, 2023
Berta Pozzi, Arunasalam Naguleswaran, Francesca Florini, Zahra Rezaei, Isabel Roditi
http://www.ebi.ac.uk/ena as study PRJEB57072. Reagents Phusion polymerase (
NEB
M0530S
); Bam HI (
NEB
R0136S
), Dpn I (
NEB
R0176S
) and Hind III (
NEB
R0104S
); Ampicilin (Sigma-Aldrich), Blasticidin (Sigma-Aldrich),
(8)
Transcription-Coupled Repair of DNA Interstrand Crosslinks by UVSSA
bioRxiv
May 13, 2023
Rowyn Church Liebau, Crystal Waters, Arooba Ahmed, Rajesh K Soni, Jean Gautier
(Abcam
ab2175
).Restriction enzymes: all restriction enzymes acquired from
NEB
:
BbsI
(
R0539S
),
BamHI
(
R0136S
),
NheI-(HF
R3131S
),
BglII
(
R0144S
),
AscI
(
R0558S
),
EcoRI
(
R0101S
)Kits: Q5
mutagenesis
kit
NEB
(
E0554S
)Equipment:
(9)
TAR Syndrome-associated Rbm8a deficiency causes hematopoietic defects and attenuates Wnt/PCP signaling
bioRxiv
May 5, 2023
Agnese Kocere, Elena Chiavacci, Kevin Manuel Méndez-Acevedo, Charlotte Soneson, Max S. Hiltabidle, Azhwar Raghunath, Jacalyn S. MacGowan, Jordan A. Shavit, Daniela Panáková, Margot L. K. Williams,
et al.
5’-TTGCTAGCTCAGCGACGTCTCCGGTCT-3’ containing a
NheI
site (underlined). The
PCR product
was cloned with
BamHI
(
R0136S
,
NEB
),
NheI
(
R3131S
,
NEB
)
restriction digest
into Tol2kit vector 396 (pCS2FA:Tol2) (Kwan et al., 2007)
(10)
A local translation program regulates centriole amplification in the airway epithelium
Scientific Reports
May 1, 2023
Helu Liu, Huijun Li, Zhihua Jiang, Shibo Jin, Rui Song, Ying Yang, Jun Li, Jingshu Huang, Xiaoqing Zhang, Xuesong Dong,
et al.
Fragment (
NEB
#
M0210S
) to blunt the end. Then EGFP fragment was released by cutting with
BamHI
(
NEB
#
R0136S
), followed by separation and
purification
of vector fragment for
ligation
. To prepare EGFP-
Tnrc6a
fragment,
(11)
Two differentially stable rDNA loci coexist on the same chromosome and form a single nucleolus
Proceedings of the National Academy of Sciences
February 28, 2023
Luciana Lazar-Stefanita, Jingchuan Luo, Max A. B. Haase, Weimin Zhang, Jef D. Boeke
chromosomal DNA (
Bio-Rad
CAT.
170-3667
). Method for
chromosome
digestion. Prior to the
BamHI
(
NEB
CAT.
R0136L
)
incubation
, agar plugs were equilibrated 1× Buffer3.1 (NEB) for 24 h at 4 °C.
BamHI
digest was carried
(12)
Structural basis for the assembly of the type V CRISPR-associated transposon complex
Cell
December 22, 2022
Michael Schmitz, Irma Querques, Seraina Oberli, Christelle Chanez, Martin Jinek
,
New England Biolabs
,Cat#
M0202S
10x
T4 DNA Ligase Reaction Buffer
,
New England Biolabs
,Cat#
B0202S
BamHI
,
New England Biolabs
,Cat#
R0136L
EcoRI-HF
®,
New England Biolabs
,Cat#
R3101
Isopropyl-β-d-thiogalactopyranoside,AG Scientific,Cat#I-1312
Carbenicillin disodium salt
(13)
Cloning BRD4 long isoform into overexpression vectors for stable overexpression of BRD4-L in mammalian cells
STAR Protocols
December 16, 2022
Ana Luiza Drumond-Bock, Magdalena Cybula, Luyao Wang, Lin Wang, Magdalena Bieniasz
antibiotic, sodium salt 25 g",VWR,Cat# 71003-352 Restriction enzyme -
BamHI
,
New England Biolabs
,Cat#
R0136S
Restriction enzyme -
EcoRI
,
New England Biolabs
,Cat#
R0101S
Restriction enzyme -
MluI
,
New England BioLabs
,Cat#
(14)
Assembly and purification of AvrSr35-induced Sr35 resistosome and determination of its structure by cryo-EM
STAR Protocols
December 16, 2022
Meng-Xi Liu, Yan-Bo Zhao, Ze-Kai Li, Tao-Tao Chen, Songying Ouyang
,
Thermo Fisher Scientific
,CAT#
EL0014
T4 DNA polymerase,
Thermo Fisher Scientific
,CAT#
18005025
BamHI
,
NEB
,CAT#
R0136S
XhoI
,
NEB
,CAT#
R0146S
SspI
,
NEB
,CAT#
R0132S
DpnI
,
NEB
,CAT#
R0176S
ATP,Sigma,CAT# 34369-07-8 MES,Sigma,CAT#
(15)
SCON-a Short Conditional intrON for conditional knockout with one-step zygote injection
Experimental & molecular medicine
December 1, 2022
Wu SS, Lee H, Szép-Bakonyi R, Colozza G, Boese A, Gert KR, Hallay N, Lee JH, Kim J, Zhu Y,
et al
.
synthesized by Genscript and subsequently cloned into the pcDNA4TO construct after digestion with
BamHI
(
R0136S
,
NEB
) and
XhoI
(
R0146S
,
NEB
) and
ligation
with T4 ligase (
M0202S
,
NEB
). The vectors were recombined
(16)
Lupus enhancer risk variant causes dysregulation of IRF8 through cooperative lncRNA and DNA methylation machinery
Nature Communications
December 1, 2022
Tian Zhou et al.
constructing pegRNA expression vector, 50946 plasmid was cut by
BbsI
(NEB, R3059L) and
BamHI
(
NEB
,
R0136S
) and then gel purified. Guide RNA oligos, gRNA scaffold oligos, RT temple and prime binding sequence
(17)
Detection of cytomegalovirus (CMV) by digital PCR in stool samples for the non-invasive diagnosis of CMV gastroenteritis
Virol J
November 11, 2022
Jia Gu, Hongyan Ji, Tongyuan Liu, Caixia Chen, Siye Zhao, Yang Cao, Na Wang, Min Xiao, Liting Chen, Haodong Cai
linearized by the restriction enzymes
HindIII
(catalog number
R0104S
;
NEB
, USA) and
BamHI
(catalog number
R0136S
;
NEB
, USA), preserving the intact CMV target sequence on the linearized plasmid. Fivefold serial dilutions
(18)
Oligodendrocyte precursor cells engulf synapses during circuit remodeling in mice
Nature Neuroscience
October 1, 2022
Yohan S. S. Auguste, Austin Ferro, Jessica A. Kahng, Andre M. Xavier, Jessica R. Dixon, Uma Vrudhula, Anne-Sarah Nichitiu, Daniele Rosado, Tse-Luen Wee, Ullas V. Pedmale,
et al.
amplified region into the hSyn–eGFP vector (Addgene, 50465) following its linearization with
BamHI
(
NEB
,
R0136S
). The resulting plasmid was gel-purified and sequenced before packaging into an adeno-associated virus
(19)
Protein purification, crystallization, and structure determination of human DEAD-box RNA helicase DDX21 in different unwinding states
STAR Protocols
September 16, 2022
Zijun Chen, Jinqing Huang, Jixi Li
Cocktail",Roche,Cat#11836170001 "
Tris
-HCI Buffer, pH 7.5 (1 M)",
Thermo Fisher Scientific
,Cat#
15567027
BamHI
,
NEB
,Cat#
R0136S
HindIII
,
NEB
,Cat#
R0104S
KOD Hot Start Master Mix
,
Merck
,Cat#
71842-3
Ulp1 protease,This paper,N/A ,, Critical
(20)
The TRESLIN-MTBP complex couples completion of DNA replication with S/G2 transition
Molecular cell
September 15, 2022
Zonderland G, Vanzo R, Gadi SA, Martín-Doncel E, Coscia F, Mund A, Lerdrup M, Benada J, Boos D, Toledo L.
Site-Directed Mutagenesis Kit,
Agilent
,Cat#
200523
"
BamHI
,
EcoRI
,
HpaI
, rCutsmart",
New England Biolabs
,"Cat#
R0136S
,
R3101S
,
R0105S
,
B6004S
"
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BamHI from New England Biolabs
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